Methods in Biophysical Chemistry
I have not yet received Dr. Lewis's slides on calorimetry, but I have included the handout he used in class, and I will email you a copy of my notes on DSC. These should be sufficient to get you through the homework assignment should you wish to start this weekend.
This week's lecture's will focus on calorimetry in biophysical applications. Friday's lecture will be given by Dr. Ed Lewis, and notes will be posted when they become available.
The materials for the NMR spectroscopy unit are included below.
- Lecture 10-3 (Intro to NMR Spectroscopy)
- Lecture 11-1 (Chemical Shifts, Scalar Couplings)
- Lecture 11-2 (Fourier Transforms, Relaxation)
- Lecture 12-2 (Assignment and Structure Determination)
- Homework #6 (NMR Spectroscopy)
As we'll see, fluorescence is a rich and diverse topic, and many interesting studies have been done on biological molecules using principles of fluorescent emission. We won't be able to cover fluorescence deeply, but I have linked a series of lectures from a workshop by Joachim Mueller at Minnesota a few years back that cover some of the same topics we will be discussing:
- Lecture 1 - Introduction
- Lecture 2 - Principles
- Lecture 3 - Instrumentation
- Lecture 4 - Quantum Yield and Polarization
- Lecture 5 - Lifetime and Polarization
- Lecture 6 - Quenching and FRET
- Lecture 7 - Fluorescence Assays
- Lecture 8 - Microscopy and FCS
You can find a link to the original workshop site here.